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Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
Author(s) -
Tsen H.Y.,
Lin C.K.,
Chi W.R.
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.853535.x
Subject(s) - escherichia coli , polymerase chain reaction , 16s ribosomal rna , enterobacteriaceae , biology , microbiology and biotechnology , oligonucleotide , gene , ribosomal rna , bacteria , genetics
The primary sequences of the V 3 and V 6 regions of the 16S rRNA gene of pathogenic and non‐pathogenic strains of Escherichia coli were determined and compared with those obtained for a number of reference strains which belong to the family Enterobacteriaceae. Three oligonucleotide primers 16E1, 16E2 and 16E3 were designed and used in the polymerase chain reaction to identify specifically all E. coli isolates. When 16E1, 16E2 and 16E3 were used as primers for the identification of E. coli cells present in tap, underground and pond waters, as low as 1 cfu 100 ml −1 of water could be detected if an 8 h pre‐culture step was performed prior to the PCR reaction.