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Identification of a gene encoding a methyl‐accepting chemotaxis‐like protein from Campylobacter coli and its use in a molecular typing scheme for campylobacters
Author(s) -
Marcos González,
Malcolm Richardson,
' Collins,
HaeSim Park
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00510.x
Subject(s) - campylobacter , typing , biology , microbiology and biotechnology , identification (biology) , chemotaxis , gene , campylobacter coli , campylobacter jejuni , genetics , escherichia coli , bacteria , computational biology , receptor , botany
Using PCR amplification with degenerate primers, a gene ( tlpA ) from Campylobacter coli encoding a putative 63·0 kDa polypeptide which exhibited significant identity with bacterial methyl‐accepting chemotaxis proteins (MCPs) was identified. A mutant containing an inactivated copy of the tlpA gene showed a wild‐type chemotactic response to all of the chemo‐attractants tested. A DNA probe based on the Highly Conserved Domain (HCD) of TlpA revealed the presence of multiple copies of genes encoding MCP‐like proteins in both Camp. coli and Camp. jejuni. The arrangement of restriction sites within, and proximal to, genes with homology to the HCD probe varied among strains, resulting in a high degree of polymorphism. It is demonstrated here that a DNA probe comprising the HCD region of MCP‐like proteins can be used, in Southern hybridization‐based assays, to provide novel information which allows the discrimination of individual strains of Camp. coli and Camp. jejuni.