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Isolation of isoproturon‐degrading bacteria from treated soil via three different routes
Author(s) -
Andrea Roberts,
Trent Walker,
Linda J. Cox,
Vivian Welch
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00507.x
Subject(s) - bacteria , degradation (telecommunications) , chemistry , biodegradation , nitrogen , enrichment culture , isolation (microbiology) , carbon fibers , urea , chromatography , environmental chemistry , microbiology and biotechnology , food science , biology , biochemistry , organic chemistry , telecommunications , genetics , materials science , composite number , computer science , composite material
Three different isolation routes (flask enrichment/flask degradation assay, flask enrichment/microplate degradation assay, MPN assay/microplate degradation assay) were used to obtain pure cultures of bacteria which degraded isoproturon (3‐(4‐isopropylphenyl)‐1,1‐dimethylurea) as sole carbon and nitrogen source in a mineral salts medium from a field soil treated with isoproturon in the laboratory. All three isolation routes were successful, but the microplate assay of degradation was more successful than the flask assay. Characterization of 36 isolates indicated that they formed 16 distinct phenotypes (10 Gram‐positive phenotypes, six Gram‐negative phenotypes) which are likely to represent distinct species. Low concentrations of the degradation product 3‐(4‐isopropylphenyl)‐1‐methylurea (IPPMU) were occasionally found in the culture solutions. When provided as the sole source of carbon and nitrogen, the monomethyl degradation product was itself rapidly degraded by several of the isolates. Some isolates were also able to use the demethylated degradation product 3‐(4‐isopropylphenyl)‐urea (IPPU) as sole source of carbon and nitrogen, although there was occasionally an extended lag‐phase before rapid degradation commenced. One isolate was particularly active and degraded isoproturon, the monomethyl and demethylated degradation products of isoproturon, and demethylated the related phenylureas diuron and linuron.