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Phospholipids of Fusobacterium spp.
Author(s) -
Sadek,
Drucker,
Boote,
Bennett,
Eley
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00506.x
Subject(s) - phosphatidylglycerol , chemistry , mass spectrometry , phosphatidylethanolamine , ion , isotope , fast atom bombardment , mass spectrum , chromatography , phospholipid , biochemistry , organic chemistry , phosphatidylcholine , physics , quantum mechanics , membrane
The aim of this study was to analyse individual polar lipid analogues, within each lipid family present, of fusobacteria using fast atom bombardment mass spectrometry (FAB‐MS). Polar lipid extracts were prepared, washed and dried. Samples, dispersed in a matrix of m ‐nitrobenzyl alcohol, were analysed by negative ion FAB‐MS using xenon as the reagent gas. Major anion peaks observed in the low mass region of mass/charge (m/z), 211, 221, 225, 227, 239, 241, 249, 251, 253, 255, 273, 277, 279, 281, 289 and 291, were consistent with the presence of C 13:1 , C 14:3 , C 14:1 , C 14:0 , C 15:1 , C 15:0 , C 16:3 , C 16:2 , C 16:1 , C 16:0 , unknown, C 18:3 , C 18:2 , C 18:1 , unknown and C 19:3 carboxylate anions. In the high mass region, major anion peaks observed with m/z 644, 646, 648, 660, 662, 672, 673, 674, 686, 688, 689, 690, 698, 700, 701, 703, 714, 716, 717 and 719 were consistent with the presence of phosphatidylethanolamine (PE) (29:2), PE (29:1), PE (29:0), PE (30:1), PE (30:0), PE (31:2), first isotope of PE (31:2), PE (31:1), PE (32:2), PE (32:1), first isotope peak of PE (32:1), PE (30:0), PE (33:3), PE (33:2), phosphatidylglycerol (PG) (31:3), PG (31:2), PE (34:2), PE (34:1), PG (32:2) and PG (32:1). We conclude that FAB‐MS can provide data on individual analogues of PE and PG from Fusobacterium spp. not readily obtained by other means. Furthermore, the phospholipid profile is diagnostic for the genus.