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Note : Lack of influence of adherent Lactobacillus isolates on the attachment of Escherichia coli to the intestinal epithelial cells of chicken in vitro
Author(s) -
Jin Jin,
Edmond S. L. Ho,
Mir Azam Khan,
Sajid Ali,
Jalaludin Jalaludin
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00434.x
Subject(s) - lactobacillus fermentum , escherichia coli , lactobacillus acidophilus , microbiology and biotechnology , in vitro , biology , lactobacillus , lactobacillaceae , enterobacteriaceae , bacteria , probiotic , lactic acid , biochemistry , genetics , gene , lactobacillus plantarum
Two Lactobacillus isolates, Lact. acidophilus I 26 and Lact. fermentum I 25, were selected, based on their poor aggregation with Escherichia coli and strong ability to adhere to ileal epithelial cells (IEC), to study in vitro interactions with E. coli O1 : K1, O2 : K1 and O78 : K80 in an IEC radioactive‐assay under the conditions of exclusion (lactobacilli and IEC, followed by the addition of E. coli ), competition (lactobacilli, IEC and E. coli together) and displacement ( E. coli and IEC, followed by the addition of lactobacilli). The results indicated that Lact. acidophilus I 26 and Lact. fermentum I 25 could not significantly reduce the attachment of E. coli O1 : K1, O2 : K1 and O78 : K80 to IEC under the three conditions tested in vitro , except that the attachment of E. coli O1 : K1 was slightly reduced by Lact. fermentum I 25 in the test for competition.