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Note : Sucrose transport and metabolism in Clostridium beijerinckii NCIMB 8052
Author(s) -
Martin Tangney,
Céline Rousse,
Mohsen Yazdanian,
Wilfrid J. Mitchell
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00432.x
Subject(s) - fructokinase , sucrose , clostridium beijerinckii , fructose , xylose , biochemistry , pep group translocation , clostridium , xylose metabolism , clostridia , invertase , chemistry , fermentation , biology , butanol , phosphoenolpyruvate carboxykinase , enzyme , bacteria , ethanol , genetics
Sucrose is the major carbon source in molasses, the traditional substrate employed in the industrial acetone‐butanol‐ethanol (ABE) fermentation by solventogenic clostridia. The utilization of sucrose by Clostridium beijerinckii NCIMB 8052 was investigated. Extracts prepared from cultures grown on sucrose (but not xylose or fructose) as the sole carbon source possessed sucrose phosphoenolpyruvate (PEP)‐dependent phosphotransferase system (PTS) activity. Extract fractionation and reconstitution experiments revealed that the entire sucrose Enzyme II complex resides within the membrane in this organism. Sucrose‐6‐phosphate hydrolase and fructokinase activities were also detected in sucrose grown cultures. The fructokinase activity, which is required specifically during growth on sucrose, was shown to be inducible under these conditions. A pathway for sucrose metabolism in this organism is proposed.