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Isolation and partial characterization of antifungal metabolites of Bacillus pumilus
Author(s) -
Munimbazi,
Bullerman
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00431.x
Subject(s) - aspergillus ochraceus , bacillus pumilus , chemistry , aspergillus fumigatus , biochemistry , chromatography , metabolite , bacillus cereus , bioassay , mycotoxin , microbiology and biotechnology , biology , ochratoxin a , bacteria , food science , genetics
Antifungal metabolites produced by Bacillus pumilus in Potato Dextrose Broth (PDB) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. The antifungal metabolites inhibited mycelial growth of many species of Aspergillus , Penicillium and Fusarium . They also inhibited production of aflatoxins, cyclopiazonic acid, ochratoxin A and patulin. The metabolites were heat‐stable and remained active after sterilization at 121 °C for 15 min. Their activity was stable over a wide range of pH (2–10). The metabolites were resistant to hydrolysis by various proteases, peptidases and other enzymes. They were also resistant to denaturation by many protein‐denaturing detergents except Nonidet P‐40. The metabolites were soluble in water and relatively polar organic solvents. Chromatographic bioassay revealed that a crude precipitate of the metabolites contained only one compound with antifungal activity. The active compound did not form a fluorescent derivative with fluorescamine suggesting that the compound is either a cyclic polypeptide or a non‐peptidic compound.