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Typing of Salmonella enteritidis of different phage types of PCR fingerprinting
Author(s) -
LópezMolina,
Laconcha,
Rementería,
Audicana,
Perales,
Garaizar
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00425.x
Subject(s) - salmonella enteritidis , serotype , salmonella , biology , phage typing , typing , microbiology and biotechnology , polymerase chain reaction , dna profiling , virology , bacteria , genetics , gene , dna
Fifty‐nine isolates of Salmonella spp. were typed by PCR fingerprinting using three single primers: ERIC2, M13 and OPS‐19. First, their discrimination power in a group of nine different serotypes were studied and considerable differences in the band patterns were obtained. Further, a panel of 51 isolates of Salmonella enteritidis with eight different phage types were analysed with the three primers. The discriminating power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types of Salm. enteritidis , but not all of them were differentiated.