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Regulation of maltose metabolism in stationary phase cultures of an asporogenous mutant of Bacillus licheniformis
Author(s) -
Tangney,
Peter J. Fleming,
Henrik L. Jørgensen,
Nicholas K. Priest
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00323.x
Subject(s) - maltose , catabolite repression , bacillus licheniformis , biochemistry , mutant , inducer , biology , fed batch culture , metabolism , enzyme , bacteria , bacillus subtilis , fermentation , genetics , gene
An asporogenous strain of Bacillus licheniformis accumulated maltose by an energy dependent transport mechanism during an extended stationary phase. Maltose transport was sensitive to the effects of the uncoupler tetrachlorosalicylanide (TCS), and was also inhibited by glucose. Maltose stimulated synthesis of a p ‐nitrophenyl‐α‐ D ‐glucoside‐hydrolysing enzyme ( p NPGase) in log phase and in stationary phase cells. In the presence of glucose this induction was inhibited. Glucose was used preferentially to maltose in stationary phase cells. The uptake of maltose from the medium, and the synthesis of p NPGase, were immediately and completely inhibited in the presence of glucose. These results are consistent with a mechanism of inducer exclusion mediating the repressive effect of glucose upon p NPGase synthesis in stationary phase cells. Catabolite repression of α‐amylase synthesis by glucose was also demonstrated in late stationary phase mutant cells.