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Establishment and impact of Pseudomonas fluorescens genetically modified for lactose utilization and kanamycin resistance in the rhizosphere of pea
Author(s) -
Naseby,
Lynch
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00321.x
Subject(s) - kanamycin , pseudomonas fluorescens , rhizosphere , alkaline phosphatase , lactose , biology , pseudomonas , amendment , bacteria , food science , microbiology and biotechnology , enzyme , biochemistry , antibiotics , genetics , law , political science
The impact of a Pseudomonas fluorescens strain, genetically modified for kanamycin resistance and lactose utilization (the GMM), could be enhanced by soil amendment with lactose and kanamycin. Lactose addition decreased the shoot to root ratio of pea, and both soil amendments increased the populations of total culturable bacteria and the inoculated GMM. Only kanamycin perturbed the bacterial community structure, causing a shift towards slower growth organisms. The community structure with the GMM inocula in the presence of kanamycin showed the only impact of the GMM compared to the wild type inocula. The shift towards K strategy (slower growing organisms), found in the other kanamycin‐amended treatments, was reduced with the GMM inoculation. Lactose amendment increased the acid and alkaline phosphatase, the phosphodiesterase activity and the carbon cycle enzyme activities, whereas the kanamycin addition only affected the alkaline phosphatase and phosphodiesterase activities. None of the soil enzyme activities was affected by the GMM under any of the soil amendments.