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Rapid estimation of spoilage bacterial load in aerobically stored meat by a quantitative polymerase chain reaction
Author(s) -
VENKITANARAYANAN K.S.,
FAUSTMAN C.,
CRIVELLO J.F.,
KHAN M.I.,
HOAGLAND T.A.,
BERRY B.W.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00368.x
Subject(s) - food spoilage , bacteria , polymerase chain reaction , biology , meat spoilage , food science , real time polymerase chain reaction , 16s ribosomal rna , microbiology and biotechnology , genetics , gene
We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemiluminescence revealed that the concentration of the amplified product was dependent on cycle number and the initial number of bacteria present in the sample. Statistical analysis of the results indicated a correlation coefficient of 0·94 ( P < 0·001) between aerobic plate count and QPCR luminosity units.