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Pulsed‐field electrophoretic fingerprinting of Salmonella indiana and its epidemiological applicability
Author(s) -
Punia,
Hampton,
; Ridley,
Michael D. Ward,
W.B. Rowe,
Threlfall
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00325.x
Subject(s) - subtyping , pulsed field gel electrophoresis , epidemiology , serotype , salmonella , typing , molecular epidemiology , outbreak , biology , genotype , environmental health , medicine , microbiology and biotechnology , genetics , virology , pathology , bacteria , computer science , gene , programming language
Eight Xba I‐generated pulsed‐field profile (PFP) types and four subtypes within one of the most common PFP types have been identified in Salmonella indiana from patients, poultry and human food in England and Wales in the three‐year period from January 1994 to December 1996. Two PFP types have predominated, PFP X1 and PFP X2. Although the PFP X1 type was identified throughout the study period, the PFP X2 type was not identified until late 1995, subsequently becoming the most common PFP type in humans in the first six months of 1996 with a significant distribution in elderly patients. It is concluded that PFGE can be used in support of epidemiological investigations for the subdivision of Salm. indiana . Furthermore, as both conditions and interpretation criteria can be easily standardized, it is suggested that for many salmonella serotypes, PFGE can provide the basis for a definitive scheme of genotypic subtyping suitable for epidemiological investigations at both a national and international level.