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Effect of carbon and nitrogen limitation on lignin peroxidase and manganese peroxidaseproduction by Phanerochaete flavido‐alba
Author(s) -
Hamman O. Ben,
De La Rubia T.,
Martinez J.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00322.x
Subject(s) - phanerochaete , lignin , manganese , manganese peroxidase , nitrogen , carbon fibers , peroxidase , chemistry , environmental chemistry , botany , biology , organic chemistry , enzyme , materials science , composite number , composite material
The ligninolytic enzymes lignin peroxidase (LiP) and manganese dependent peroxidase(MnP), were detected in extracellular fluids of Phanerochaete flavido‐alba FPL 106507cultures under carbon or nitrogen limitation. MnP activities were found to be higher than LiPactivities under all growth conditions tested. Higher titres of both peroxidases were obtainedunder carbon limitation in excess nitrogen. Isoelectric points (pIs) observed after FPLC and IEFof concentrated extracellular fluids revealed more acidic pIs for LiP enzymes obtained innitrogen‐limited cultures than those in carbon‐limited cultures. However, the change in thelimiting growth factor does not significantly affect MnP pIs.