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Characterization of the bacterial flora of Sudanese sorghum flour and sorghum sourdough
Author(s) -
Hamad S.H.,
Dieng M.C.,
Ehrmann M.A.,
Vogel R.F.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00310.x
Subject(s) - biology , sorghum , lactobacillus reuteri , lactobacillus fermentum , microbiology and biotechnology , lactobacillus , 16s ribosomal rna , food science , bacteria , lactococcus , fermentation , lactococcus lactis , lactic acid , lactobacillus plantarum , genetics , ecology
The microflora of a Sudanese sorghum flour, a spontaneously fermented sourdough and along‐term sourdough produced in a Sudanese household by consecutive re‐inoculations, wasstudied. The dominant contaminants of sorghum flour were Gram‐negative, catalase‐positive,rod‐shaped bacteria with counts of about 10 5 cfu g −1 . Thespontaneously fermented sorghum sourdough showed a bacterial succession from Gram‐negative,catalase‐positive contaminants to Enterococcus faecalis , Lactococcus lactis , Lactobacillus fermentum and Lact. reuteri . The total bacterial countreached about10 10 cfu g −1 and the pH dropped from6·4 to 3·35 in about 42 h. In this phase, only the latter two species remaineddominant in a ratio of 1:1. From the Sudanese long‐term dough, seven strains of Lactobacillus were isolated, representing the dominant flora. Sequence comparison ofpartial 16S rRNA gene sequences were used to clarify their phylogenetic positions. Five strainswere classified as Lact.vaginalis and could be regarded as heterogenous biovars of thisspecies. The other two strains could be assigned to Lact. helveticus .RAPD‐PCR and sugar fermentation patterns were useful in differentiation of these strains.