Premium
Characterization of the aggregation promoting factor from Lactobacillus gasseri , avaginal isolate
Author(s) -
Boris S.,
Suárez J.E.,
Barbés C.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00250.x
Subject(s) - lactobacillus gasseri , proteases , microbiology and biotechnology , lactobacillus plantarum , enterococcus faecalis , lactobacillus , biochemistry , biology , chemistry , bacteria , enzyme , escherichia coli , lactic acid , fermentation , gene , genetics
Lactobacillus gasseri 2459, isolated from the human vagina, exhibits a strongautoaggregating phenotype. Filter‐sterilized spent supernatants of this strain promote aggregationof Lact. plantarum LL441 and Enterococcus faecalis EF. Aggregation wasabolished upon exposure of the cells to proteases and, in the case of Ent. faecalis , tometaperiodate, which suggests the involvement of cell‐surface proteins and glycoproteins,respectively, in the aggregation phenotype. In accordance with this, a 75 kDa surface protein, andpossibly another of approximately 94 kDa, appears in Lact. plantarum LL441 culturesincubated with Lact. gasseri culture supernatants. The diffusible aggregation promotingfactor was purified from stationary phase culture supernatants and determined to be a 2 kDahydrophilic peptide active at pH 3–4 and stable at neutral and acid pH. The activity wasresistant to heat, chymotrypsin, chelating agents, triton X‐100 and reducing agents, but sensitiveto other proteases and SDS.