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Use of the Malthus conductance growth analyser to determine numbers of thermophilic streptococci on stainless steel
Author(s) -
Flint S.H.,
Brooks J.D.,
Bremer P.J.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00233.x
Subject(s) - analyser , acridine orange , conductance , streptococcus bovis , microbiology and biotechnology , fluorescence microscope , fluorescence , biology , bacteria , thermophile , food science , chemistry , chromatography , biochemistry , optics , genetics , apoptosis , rumen , physics , mathematics , combinatorics , fermentation
The use of the Malthus conductance growth analyser for the detection of Streptococcus bovis attached to stainless steel surfaces was evaluated. A comparison between the results from acridine orange epifluorescence direct counts, swab recovery viable count and conductance estimates of attached cell concentrations, based on calibrations for planktonic cells, showed that the conductance results were up to 2 log 10 greater than the epifluorescence results and the swab counts. The growth rates of planktonic and attached cells were similar over 16 h using the Malthus technique. This suggests that the Malthus technique detects more attached cells of Strep. bovis than epifluorescence microscopy or swab recovery.