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Detection, distribution and probable fate of Escherichia coli O157 from asymptomatic cattle on a dairy farm
Author(s) -
Porter J.,
Mobbs K.,
Hart C.A.,
Saunders J.R.,
Pickup R.W.,
Edwards C.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00230.x
Subject(s) - immunomagnetic separation , escherichia coli , biology , feces , microbiology and biotechnology , enterobacteriaceae , food science , biochemistry , gene
The use of commercial anti‐ Escherichia coli O157‐labelled magnetic beads was investigated to improve detection of E. coli O157 by immunomagnetic separation (IMS) from a range of environments on a dairy farm. Immunomagnetic separation proved effective for separation of target cells from laboratory mixtures and during stress in sterile and non‐sterile pond water. The IMS procedure was possible with a range of samples (water, faeces, slurry, grass and soil). Non‐specific binding of non‐target bacterial cells proved problematic in a number of sample types. However, indigenous E. coli O157 cells were detected from samples with a high faecal load, and only with use of IMS. Data on the probable survival and spread of the organism around the farm environment are also discussed.