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Optimization of D ‐ribose production with a transketolase‐affected Bacillus subtilis mutant strain in glucose and gluconic acid‐based media
Author(s) -
De Wulf P.,
Soetaert W.,
Schwengers D.,
Vandamme E.J.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00161.x
Subject(s) - acetoin , gluconic acid , fermentation , transketolase , bacillus subtilis , biochemistry , ribose , chemistry , food science , substrate (aquarium) , bacteria , biology , enzyme , genetics , ecology
When the transketolase‐deficient and D ‐ribose‐producing Bacillus subtilis strain ATCC 21951 was grown in a glucose (200 g l −1 )‐based medium (Kintaka et al. 1986), only 11 g l −1 D ‐ribose was synthesized, in addition to acetic acid (12 g l −1 ) and acetoin plus 2,3‐butanediol (24 g l −1 ), within 1 week of fermentation. After optimizing the process conditions at 2 l fermentor scale (simplified medium composition, pH 5·0 or 6·0, highly oxidative (1000 rev min −1 , 3 vvm)), 40 g l −1 D ‐ribose was obtained from 200 g l −1 D ‐glucose, in addition to 14·5 g l −1 acetoin, during 1 week of fermentation. By partially substituting D ‐glucose with D ‐gluconic acid (100 g l −1 D ‐glucose plus 50 g l −1 D ‐gluconic acid) under highly oxidative (1000 rev min −1 , 3 vvm) and pH‐controlled (pH 6·5) conditions, D ‐ribose productivity increased (45 g l −1 ) and acetoin formation (7·5 g l −1 ) dropped, as did the fermentation time (3·5 d). The mixed carbon substrate procedure here developed provides an excellent alternative to the less efficient glucose‐based processes described so far.