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Isolation and characterization of refuse methanogens
Author(s) -
Ladapo J.A.,
Barlaz M.A.
Publication year - 1997
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1997.00154.x
Subject(s) - mesophile , bacteria , isolation (microbiology) , microbiology and biotechnology , lysis , sewage , sewage sludge , biology , chemistry , yeast extract , food science , sodium , fermentation , biochemistry , waste management , genetics , organic chemistry , engineering
Four mesophilic, irregular, rod‐shaped methanogenic bacteria were isolated from decomposing refuse recovered from laboratory‐scale reactors and a municipal solid waste landfill. H 2 /CO 2 was the only substrate on which the isolates could grow in a complex medium. Isolates grew between either 25° or 30° and 45°C and between pH 6 and 8. One isolate exhibited growth at pH 5. Growth of each isolate was enhanced by yeast extract and inhibited by anaerobic sewage sludge supernatant fluid. No isolate showed greater than 25% lysis on exposure to 1% sodium dodecyl sulphate (SDS) for 24 h, as is typical of methanogens with a proteinaceous cell wall. The physiological traits of the methanogens isolated here are similar to many previously characterized isolates.