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ESR detection of irradiated broad bean ( Vicia faba L.) and kinetics of the radiation induced free radical and Mn 2+ signals
Author(s) -
Polat Mustafa,
Korkmaz Mustafa
Publication year - 2003
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1046/j.1365-2621.2003.00718.x
Subject(s) - electron paramagnetic resonance , radical , irradiation , kinetics , annealing (glass) , analytical chemistry (journal) , chemistry , vicia faba , radiolysis , radiation , atmospheric temperature range , radiation chemistry , ion , nuclear magnetic resonance , radiochemistry , materials science , nuclear physics , physics , botany , quantum mechanics , meteorology , composite material , biology , chromatography , organic chemistry
Summary An ESR (Electron Spin Resonance) investigation on irradiated dry broad bean gave a spectrum which was composed of an equally spaced sextet and a single resonance line. These lines appeared at g = 2.0045 (±0.0005) and originated from Mn 2+ ions and radiation induced radicals, respectively. Ground broad bean was used throughout the work to avoid any artefacts arising from a microwave cavity filling factor. Free radical signal intensity was observed to increase exponentially in relation to the increase in absorbed dose over the dose range 1.25–15 kGy. Although the Mn 2+ signal increases below room temperature, the signal due to the natural and radiation induced radicals decreases. Above room temperature, they both decreased and these decreases were irreversible. The kinetics of these decreases were studied in detail over a temperature range of 308–373 K by annealing samples at different temperatures for various times.