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Inhibition of endive ( Cichorium endivia L.) polyphenoloxidase by a Carica papaya latex preparation
Author(s) -
De Rigal David,
Cerny Muriel,
RichardForget Florence,
Varoquaux Patrick
Publication year - 2001
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1046/j.1365-2621.2001.00498.x
Subject(s) - chemistry , carica , size exclusion chromatography , sephadex , chromatography , enzyme , ion chromatography , biochemistry , botany , biology
When endive polyphenoloxidase (PPO) was incubated with a crude papaya latex extract, it rapidly lost its activity. Inactivation was ascribed to thermostable nonenzymatic factors of low molecular weight. These factors were partially purified by a two step protocol including gel filtration chromatography on Biogel P2 and ion exchange chromatography using DEAE Sephadex A25. The PPO‐inactivation rate was first order, when either inactivating agent or proton concentration was evaluated. Inactivation could be partially reversed by CuSO 4 , which suggested that the inactivating factor(s) bound to the copper site of the enzyme. On a more rapid time scale than inactivation, papaya latex extract acted also as a weak noncompetitive PPO inhibitor.