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A modified colorimetric method for the estimation of β‐cyclodextrin using phenolphthalein
Author(s) -
Basappa Channa,
Rao Pramila,
Rao D. Narasimha
Publication year - 1998
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1046/j.1365-2621.1998.00216.x
Subject(s) - phenolphthalein , cyclodextrin , absorbance , chemistry , chromatography , logarithm , analytical chemistry (journal) , mathematics , organic chemistry , mathematical analysis
The binding equilibrium between β‐cyclodextrin and phenolphthalein has been used to develop a method for the estimation of β‐cyclodextrin in solution. From logarithmic plots of amounts of β‐cyclodextrin against absorbance at 554 nm, a relation log X = (log A − log Y )/ B was found, which gave an estimate of β‐cyclodextrin in the concentration range 0.0045 mg mL −1 (3.96 μm) to 4.7 mg mL −1 (4.14 mm), where X = intercept and B = slope. This method was found to be highly reproducible and reliable.