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Effect of various doses of palm vitamin E and tocopherol on aspirin‐ induced gastric lesions in rats
Author(s) -
Jaarin Kamsiah,
Gapor M.T.,
Nafeeza M.I.,
Fauzee A.M.
Publication year - 2002
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.2002.00242.x
Subject(s) - vitamin e , aspirin , palm , vitamin , malondialdehyde , mucus , meal , tocopherol , alpha tocopherol , stomach , zoology , chemistry , medicine , food science , antioxidant , biology , biochemistry , ecology , physics , quantum mechanics
Summary. This study examined the effects of vitamin E on the prevention of aspirin‐induced gastric lesions. The study was divided into two phases. Phase 1 determined the effects of various doses of palm vitamin E on the factors affecting mucosal integrity. Thirty‐two male rats of the Sprague‐Dawley strain (200–250 g) were randomly divided into four groups. Group I was fed a normal diet (control), Groups II, III and IV were fed a diet supplemented with palm vitamin E in a dose of 60 mg/kg food, 100 mg/kg food and 150 mg/kg food, respectively. The rats were killed after 4 weeks of feeding for the determination of gastric malondialdehyde (MDA), acid and mucus content. There was a significant decrease in gastric MDA and gastric acid in all the palm vitamin E supplemented groups compared to control. However, these doses of palm vitamin E had no significant effect on gastric mucus. The phase 2 study determined the effect of multiple doses of palm vitamin E and tocopherol on the prevention of aspirin‐induced gastric lesions. Fifty rats of the same weight and strain were randomized into seven groups. Group I was fed a normal diet; groups II to IV were fed with a palm vitamin E enriched diet in doses of 60 mg, 100 mg and 150 mg/kg food, respectively; groups V to VII were fed with a tocopherol‐enriched diet in doses of 20 mg, 30 mg and 50 mg/kg food, respectively. After 4 weeks of feeding with the respective diets the rats were challenged with a single intra‐gastric dose of 400 mg/kg body weight aspirin suspended in propylene glycol. The rats were killed 6 h post‐aspirin exposure for the determination of gastric lesion index and gastric parameters as mentioned in the phase I study. The gastric lesions index was significantly lower in all the vitamin E groups compared to control. The lowest ulcer index was observed in the groups that received 100 mg of palm vitamin E and 30 mg tocopherol in the diet. However, there was no significant difference in ulcer indices between palm vitamin E and tocopherol‐treated groups. The lower ulcer index was only accompanied by lower gastric MDA content. We conclude that both palm vitamin E in doses of 60 mg, 100 mg and 150 mg/kg food as well as tocopherol in doses of 20 mg, 30 mg and 50 mg/kg food are equally effective in preventing aspirin‐induced gastric lesions. The most probable mechanism is through their ability in limiting the lipid peroxidation that is involved in aspirin‐induced gastric lesions.