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Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon‐γ production
Author(s) -
LagrotaCandido Jussara,
Vasconcellos Rita,
Cavalcanti Marta,
Bozza Marcelo,
Savino Wilson,
QuiricoSantos Thereza
Publication year - 2002
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.2002.00221.x
Subject(s) - lymph , duchenne muscular dystrophy , mdx mouse , muscular dystrophy , biology , inflammation , germinal center , skeletal muscle , pathology , lymph node , itga7 , immunology , endocrinology , medicine , dystrophin , antibody , b cell
Summary. Mdx mouse, the animal model of Duchenne muscular dystrophy, develops an X‐linked recessive inflammatory myopathy with an apparent sustained capacity for muscle regeneration. We analysed whether changes in the skeletal muscle during myonecrosis and regeneration would correlate with functional alterations in peripheral lymphoid tissues. Here we show that during the height of myonecrosis, mdx mice display marked atrophy of peripheral lymph nodes and extensive muscle inflammation. In contrast, enlargement of draining lymph nodes with accumulation of CD4 + CD44 + , CD4 + CD25 + , CD8 + CD44 + T lymphocytes and type‐2 B cells was consistently observed during amelioration of the muscle lesion. In addition, regeneration of the muscular tissue was accompanied by concomitant increase of immunoglobulin‐secreting cells in regional lymph nodes and bone marrow. Double immunolabelling analysis revealed intense B cell proliferation and formation of germinal centre in the follicles of dystrophic regional lymph nodes. Furthermore, lymph node cells produced large amounts of IFN‐γ but not IL‐4, IL‐6 or IL‐10 after in vitro mitogen stimulation with Concanavalin A. As these alterations occurred mainly during the recovery period, we suggested that local activation of the immune system could be an influence which mitigates the myonecrosis of muscular tissue in the mdx dystrophic mouse.