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Effects of vitamin E on human platelet and mononuclear cell responses in vitro
Author(s) -
Williams J.C.,
Forster L.A.,
Tull S.P.,
Ferns G.A.A.
Publication year - 1999
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1999.00118.x
Subject(s) - platelet , incubation , peripheral blood mononuclear cell , adhesion , in vitro , vitamin e , thrombin , vitamin , chemistry , medicine , cell adhesion , endothelial stem cell , endocrinology , andrology , immunology , biochemistry , cell , antioxidant , organic chemistry
Recent epidemiological studies have provided evidence supporting the potential benefits of antioxidants in coronary prevention. We have investigated the effects of vitamin E on platelets, monocytes and endothelial cells in vitro . Pre‐incubation of platelets with vitamin E inhibited subsequent thrombin‐ ( P < 0.05, n = 5), collagen‐ ( P < 0.0001, n = 5) and ADP‐( P < 0.05, n = 4) induced platelet aggregation measured using a microtitre plate method, or conventional aggregometry. The adhesion of thrombin‐activated platelets to collagen was also inhibited by vitamin E ( P < 0.05, n = 8), but not by vitamin C ( P > 0.05, n = 8); nor was the adhesion of unstimulated platelets significantly affected ( P > 0.05, n = 8). Pre‐incubation of monocytes with vitamin E inhibited their subsequent adhesion to plastic ( P < 0.05, n = 9), and was also associated with an 18% reduction in adhesion to EA.hy 926 endothelial cells ( n = 8), although this failed to reach statistical significance. Pre‐incubation of the endothelial cells with vitamin E also significantly reduced subsequent mononuclear cell adhesion by 56% ( P < 0.05, n = 3).