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Tissue distribution of rat macroglobulins in tumour‐bearing rats
Author(s) -
Н. В. Мальцева,
Р. М. Зорина,
N V Mingaljev,
N A Krajushkina,
Т. С. Чирикова,
Н. А. Зорин
Publication year - 1999
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1999.00103.x
Subject(s) - immunoelectrophoresis , spleen , globulin , endocrinology , distribution (mathematics) , blood proteins , lung , macroglobulin , medicine , chemistry , biology , pathology , antibody , immunology , mathematical analysis , mathematics
Rat macroglobulins were determined in blood sera and extracts of tissues of intact rats and rats with Walker carcinoma by rocket immunoelectrophoresis. The serum levels of α 1 ‐macroglobulin (α 1 MG) α 2 ‐macroglobulin (α 2 MG) and pregnancy‐associated α 1 ‐glycoprotein (α 1 PAG) were 1.86 ± 0.07 mg/ml, 0.12 ± 0.02 mg/ml and 18.32 ± 4.07 AU/ml respectively in control rats. Maximum concentrations of α 1 MG were found in heart, lung and spleen and lesser quantities were in liver and thymus, while α 2 MG and α 1 PAG were not found at all in tissue extracts from control rats. Serum α 2 MG and α 1 PAG concentrations increased more than 30‐fold in tumour‐bearing rats compared to control animals, while α 1 MG serum concentration was little changed. Increases in all three macroglobulins occurred in the tissues of tumour‐bearing rats, particularly α 1 PAG. The tissue concentrations of α 1 MG and α 2 MG were similar and the tissue distribution was also similar with highest concentrations in heart and lung. Considerable quantities of the proteins were found in the tumour and part of peritoneum which made contact with the tumour. Changes in the protein concentrations in serum and tissue extracts of tumour‐bearing rats suggest that all members of rat macroglobulin family are disturbed during the development of the Walker carcinoma, though only α 2 MG and α 1 PAG were substantially elevated.