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The bcl‐2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype
Author(s) -
BOOTHANDFORD R. P.,
MICHAELIDIS T. M.,
HILLARBY M. C.,
ZAMBELLI A.,
DENTON J.,
HOYLAND J. A.,
FREEMONT A. J.,
GRANT M. E.,
WALLIS G. A.
Publication year - 1998
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1998.790411.x
Subject(s) - endochondral ossification , osteoblast , knockout mouse , phenotype , ossification , endocrinology , bone growth , biology , medicine , anatomy , cartilage , in vitro , genetics , receptor , gene
Histological examination of long bones from 1‐day‐old bcl‐2 knockout and age‐matched control mice revealed no obvious differences in length of bone, growth plate architecture or stage of endochondral ossification. In 35‐day‐old bcl‐2 knockout mice that are growth retarded or ‘dwarfed’, the proliferative zone of the growth plate appeared slightly thinner and the secondary centres of ossification less well developed than their age‐matched wild‐type controls. The most marked histological effects of bcl‐2 ablation were on osteoblasts and bone. 35‐day‐old knockout mouse bones exhibited far greater numbers of osteoblasts than controls and the osteoblasts had a cuboidal phenotype in comparison with the normal flattened cell appearance. In addition, the collagen deposited by the osteoblasts in the bcl‐2 knockout mouse bone was disorganized in comparison with control tissue and had a pseudo‐woven appearance. The results suggest an important role for Bcl‐2 in controlling osteoblast phenotype and bone deposition in vivo .