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Expression of heat shock protein 47 is increased in remnant kidney and correlates with disease progression
Author(s) -
SUNAMOTO MASAAKI,
KUZE KOGO,
IEHARA NORIYUKI,
TAKEOKA HIROYA,
NAGATA KAZUHIRO,
KITA TORU,
DOI TOSHIO
Publication year - 1998
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1998.00061.x
Subject(s) - glomerulosclerosis , extracellular matrix , heat shock protein , in situ hybridization , focal segmental glomerulosclerosis , kidney , messenger rna , kidney disease , pathology , nephrectomy , biology , endocrinology , medicine , glomerulonephritis , microbiology and biotechnology , proteinuria , biochemistry , gene
Glomerulosclerosis is characterized by accumulation of the mesangial extracellular matrix, including type I and IV collagen. The processing for the collagens in the glomeruli may play a critical role for development of glomerulosclerosis. We examined the expression of heat shock protein 47 (HSP47), a collagen‐binding molecular chaperone in the progresive glomerulosclerosis model. Subtotally nephrectomized rats, unlike sham‐operated rats, developed focal and segmental glomerulosclerosis. Immunological staining demonstrated an increased expression of HSP47 which paralleled the expression of type I and IV collagen in the glomeruli of the nephrectomized rats as the glomerulosclerosis developed. The mRNA levels encoding type I and type IV collagen and HSP47 were increased 3.4 fold, 3.6 fold and 2.8 fold, respectively, at week 7 after nephrectomy. By in situ hybridization, the expression of HSP47 mRNA was determined to be localized to the glomeruli with segmental sclerosis. These results suggest that HSP47 may play a central role in the process of extracellular matrix accumulation during the development of glomerulosclerosis.