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Modification of spontaneous renal lesion of APA hamsters by streptozotocin‐induced diabetes
Author(s) -
NISHIDA ETSUKO,
YAMANOUCHI JUN,
OGATA SEIYA,
DOI KUNIO,
ITAGAKI SHINICHI
Publication year - 1997
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1997.240354.x
Subject(s) - streptozotocin , glomerular basement membrane , lesion , immunohistochemistry , diabetes mellitus , pathology , renal capsule , hamster , glomerulosclerosis , basement membrane , medicine , endocrinology , podocyte , glomerulonephritis , kidney , proteinuria
Syrian hamsters of the APA strain (APA hamsters) develop spontaneous mesangial thickening in the renal glomeruli from an early age. They also develop focal and segmental glomerulosclerosis (FSG) at and after 6 months of age. In this study, histopathological, immunohistochemical and lectin histochemical examinations were conducted to clarify the modification of the spontaneous renal lesions of APA hamsters by streptozotocin(SZ)‐induced diabetes. Histopathological analysis revealed that the expansion of the mesangial region was more prominent and the thickening of the glomerular basement membrane (GBM) was weaker in SZ‐treated animals than in non‐treated ones. Immunohistochemical analysis suggested that type IV collagen and laminin were involved in the expansion of the mesangial region and thickening of the GBM. In lectin histochemical analysis, podocytes, capillary endothelial cells, GBM and a part of mesangial region of SZ‐treated animals were positive for RCA 120 and GSL‐I with neuraminidase‐pretreatment although they were negative for these lectins in non‐treated animals. These results suggest that the spontaneous glomerular lesion of APA hamsters is modified qualitatively and quantitatively by SZ‐induced diabetes.