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Dengue virus‐induced cytotoxin releases nitrite by spleen cells
Author(s) -
MUKERJEE RUMA,
MISRA ANITA,
CHATURVEDI U.C.
Publication year - 1996
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1996.962100.x
Subject(s) - dengue fever , dengue virus , virology , spleen , virus , nitrite , biology , microbiology and biotechnology , immunology , ecology , nitrate
Dengue type‐2 virus (DV) infection in mice induces T cells to produce a cytokine, the cytotoxic factor (CF), which induces H2‐A positive macrophages to produce another cytokine, cyotoxin (CF2), which amplifies its cytotoxic effects on target cells. The present study was undertaken to investigate the production of nitrite (NO − 2 ) by the spleen cells of mice in vitro and in vivo following inoculation of CF2. Maximum NO − 2 production occurred at 1 hour after inoculation of 100 μg CF2. Pretreatment of CF2 with anti‐CF2‐antisera (CF2‐As) inhibited the production of NO − 2 . Pretreatment of the spleen cells with N G ‐monomethyl‐ l ‐arginine (NMA) or with arginase inhibited NO − 2 production. The NO − 2 production was diminished in a dose dependent manner by treatment of spleen cells with the Ca 2+ channel blocking drug, nifedipine and Zn 2+ as ZnSO 4 . The findings of the present study thus demonstrate that CF2 induces production of NO − 2 in the spleen cells in a CA 2+ ‐dependent manner which may be a mechanism of target cell killing.