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Insulin‐like growth factor‐I modulates monocyte adhesion to EAhy 926 endothelial cells
Author(s) -
MOTANI ALYKHAN,
FORSTER LOUISE,
TULL SAMANTHA,
ÄNGGÅRD ERIK E.,
FERNS GORDON A.A.
Publication year - 1996
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1996.960098.x
Subject(s) - monocyte , nitric oxide , growth factor , endothelial stem cell , angiogenesis , endothelium , endocrinology , medicine , chemistry , cell adhesion , microbiology and biotechnology , biology , cell , immunology , in vitro , biochemistry , receptor
IGF‐I is a ubiquitous growth factor, found in platelets and elaborated by many other cell types. It is thought to be involved in several pathophysiological processes including embryonic development, angiogenesis and wound healing. We report that the adherence of human peripheral blood monocytes to an endothelial cell line (EAhy 926) is inhibited in a dose and time‐dependent manner by pre‐incubating the endothelial cells with IGF‐I ( P < 0.001). Monocyte adhesion was inhibited 17.9 ± 1.9% by IGF‐I at a dose of 1000 ng/ml ( P < 0.01). In contrast, IGF‐I had no significant effect on monocyte adherence to plastic. The inhibitory effects of IGF‐I were reversed by co‐incubating the endothelial cells with the nitric oxide synthase inhibitor, L‐NAME. These data suggest that the effects of IGF‐I are mediated by the release of nitric oxide from the endothelial cells.