Premium
Relation between distribution of viral RNA and development of histopathological changes in encephalomyocarditis virus‐induced orchitis in mice
Author(s) -
UENO AITO,
TAKEDA MAKIO,
HIRASAWA KENSUKE,
ITAGAKI SHIN’ICH,
DOI KUNIO
Publication year - 1996
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1046/j.1365-2613.1996.959097.x
Subject(s) - sertoli cell , germinal center , virus , biology , rna , in situ hybridization , orchitis , virology , pathology , microbiology and biotechnology , immunology , spermatogenesis , endocrinology , messenger rna , b cell , medicine , antibody , biochemistry , gene
The relation between the distribution of viral RNA and the development of histopathological changes was investigated in the early stage of encephalomyocarditis (EMC) virus‐induced orchitis in mice. Signals of viral RNA were first detected by in situ hybridization in a few Sertoli cells in almost intact germinal epithelia at 2 days post‐inoculation (d.p.i.). The number of Sertoli cells bearing signals of viral RNA increased at 3 d.p.i. when mild degenerative changes were exceptionally found in germinal epithelia. Signals of viral RNA came to be detected not only in Sertoli cells but also in a small number of germinal cells and spermatogonia at 4 d.p.i. when mild to moderate degenerative changes developed in germinal epithelia, resulting in desquamation of degenerated cells. At the same time, virus‐like particles were observed by electron‐microscopy in the degenerated and desquamated germinal cells. At and after 5 d.p.i., luminal obstruction with cellular debris and inflammatory cells was generally found. These results suggest that EMC virus carried to seminiferous tubules via the blood first attacks Sertoli cells and then damages germinal cells and spermatogonia.