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Induction of interleukin‐6 gene expression by pro‐inflammatory cytokines and black‐pigmented Bacteroides in human pulp cell cultures
Author(s) -
Yang L.C.,
Tsai C.H.,
Huang F.M.,
Liu C.M.,
Lai C.C.,
Chang Y.C.
Publication year - 2003
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.2003.00663.x
Subject(s) - bacteroides , prevotella intermedia , porphyromonas gingivalis , pulp (tooth) , gene expression , tumor necrosis factor alpha , biology , interleukin , microbiology and biotechnology , inflammation , immunology , gene , cytokine , medicine , pathology , bacteria , genetics
Aim To investigate the effect of pro‐inflammatory cytokines and black‐pigmented Bacteroides on the expression of IL‐6 gene in human pulp fibroblasts. Methodology IL‐1α, tumour necrosis factor‐α (TNF‐α) and the supernatants of Porphyromonas endodontalis , P. gingivalis and Prevotella intermedia were used to evaluate IL‐6 gene expression in human pulp fibroblasts. The levels of mRNAs were measured by the quantitative reverse‐transcriptase polymerase chain reaction analysis. Results Investigations of the time dependence of IL‐6 mRNA expression in pro‐inflammatory cytokines‐treated cells revealed a rapid accumulation of the transcript after 2 h of exposure and remained elevated throughout the 24‐h incubation period. In addition, black‐pigmented Bacteroides also induced IL‐6 gene expression in human pulp fibroblasts. Conclusions Pro‐inflammatory cytokines and black‐pigmented Bacteroides may be involved in developing pulpal inflammation through the stimulation of IL‐6 production.