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Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers
Author(s) -
Miletić I.,
Jukić S.,
Anić I.,
Željezić D.,
GarajVrhovac Osmak V., M.
Publication year - 2003
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.2003.00647.x
Subject(s) - micronucleus test , cytotoxic t cell , chemistry , cytotoxicity , micronucleus , incubation , in vitro , hamster , microbiology and biotechnology , biology , toxicity , biochemistry , organic chemistry
Aim To study in vitro the cytotoxic and mutagenic effects of AH26 and AH Plus. Methodology Cytotoxic effects on Chinese hamster V79 cells were determined by counting viable cells following incubation with eluations of AH26 and AH Plus. In one set of experiments, the materials were mixed, set for 1 h and then eluted with dimethyl sulphoxide (DMSO) for 1 h, 24 h and 7 days. In the other set, AH26 and AH Plus were mixed and set for 1 h, 24 h and 7 days in physiological saline then crushed and eluted in DMSO for 24 h. The cytotoxic effects of these eluates were evaluated. Three concentrations were chosen to examine the mutagenic effects of AH26 and AH Plus: 5.57, 16.7 and 55.7 μg mL −1 . The structural chromosomal aberration analysis and micronucleus test were performed on human lymphocytes according to standard procedures. Results Dose–response curves of cell survival were obtained. Both materials were shown to be cytotoxic in doses larger than 55.7 μg mL −1 , except for AH26, after 7 days setting time. AH Plus was also shown to be toxic in concentrations of 16.7 μg mL −1 , except after 7 days setting time. Neither AH26 nor AH Plus induced a significant increase of chromosomal aberrations or micronuclei induction at any setting time or concentration. Conclusion There was no mutagenicity found for AH26 and AH Plus on human lymphocytes in highly controlled conditions in vitro.