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Polymerase chain reaction detection of Treponema denticola in endodontic infections within root canals
Author(s) -
Siqueira J. F.,
Rôças I. N.,
Favieri A.,
Oliveira J. C. M.,
Santos K. R. N.
Publication year - 2001
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.2001.00382.x
Subject(s) - treponema denticola , polymerase chain reaction , 16s ribosomal rna , microbiology and biotechnology , dentistry , biology , medicine , periodontitis , gene , genetics , porphyromonas gingivalis
Aim A 16S rDNA‐based polymerase chain reaction (PCR) method was used to detect the occurrence of Treponema denticola in root canals. Methodology Samples were collected from 54 single‐rooted teeth having carious lesions and necrotic pulps. DNA extracted from the samples was amplified using the PCR assay, which yielded a specific fragment of T. denticola 16S rDNA. Results T. denticola was detected in 10 of 29 asymptomatic cases (34.5%), eight of 15 root canals that were tender to percussion (53.3%), and five of 10 teeth with acute periradicular abscess (50%). In general, T. denticola was found in 23 of 54 cases (42.6%). Conclusions Findings suggest that T. denticola can be involved in the pathogenesis of periradicular lesions of endodontic origin.