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TGF‐β1 alone and in combination with calcium hydroxide is synergistic to TGF‐β1 production by osteoblasts in vitro
Author(s) -
Jaunberzins A.,
Gutmann J. L.,
Witherspoon D. E.,
Harper R. P.
Publication year - 2000
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.2000.00326.x
Subject(s) - osteoblast , subculture (biology) , calvaria , medicine , endocrinology , autocrine signalling , transforming growth factor beta , alkaline phosphatase , chemistry , in vitro , transforming growth factor , biology , biochemistry , microbiology and biotechnology , receptor , enzyme
Aim To examine the effects of calcium hydroxide (Ca(OH) 2 ), transforming growth factor‐beta (TGF‐β1), and Ca(OH) 2 /TGF‐β1 coadministration on TGF‐β1 and interleukin‐6 (IL‐6) synthesis by early (subculture 1) and late (subculture 5) osteoblast cultures. Methodology Early and late cultures were established using bone cells harvested from 21‐day‐old fetal rat calvaria. Cell cultures of both early and late osteoblasts were divided into four groups: group 1, control; group 2, cells challenged with Ca(OH) 2 ; group 3, cells challenged with TGF‐β1; and group 4, cells challenged with Ca(OH) 2 and TGF‐β1 in combination. TGF‐β1 and IL‐6 levels for all groups were determined using ELISA methodology. Results anova and Tukey HS analyses revealed that osteoblasts of groups 3 and 4 significantly increased ( P < 0.001) TGF‐β1 synthesis in both early and late cultures of osteoblasts. IL‐6 was not detected in any of the groups considered in this study. Conclusions Exogenous TGF‐β1 has an autocrine effect on cell cultures of osteoblasts. Administration of TGF‐β1 alone or in combination with Ca(OH) 2 increases the synthesis of TGF‐β1 in osteoblast cultures. Ca(OH) 2 and TGF‐β1 are compatible when placed in a culture of osteoblasts. Ca(OH) 2 provides a favourable environment for the anabolic effects of TGF‐β1.