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Effects of alternating and direct electrical current application on the odontoblastic layer in human teeth: an in vitro study
Author(s) -
AlwasDanowska H. M.,
Huysmans M.C. D. N. J. M.,
Verdonschot E. H.
Publication year - 1999
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.1999.00245.x
Subject(s) - odontoblast , alternating current , direct current , haematoxylin , materials science , dentistry , smear layer , biomedical engineering , medicine , pulp (tooth) , dentin , staining , pathology , voltage , physics , quantum mechanics
Alwas‐Danowska HM, Huysmans M‐CDNJM, Verdonschot EH . Effects of alternating and direct electrical current application on the odontoblastic layer in human teeth: an in vitro study. International Endodontic Journal , 32, 459–463 1999. Aim The aim of this study was to investigate the influence of a low intensity alternating current on the odontoblasts and odontoblast layer and compare this with the effects of a direct current. Methodology Teeth extracted for orthodontic reasons were immersed in physiological saline stabilized with thymol crystals. Within 1h of extraction, an alternating or direct current was applied on the crown in the direction of the apex of the tooth for 120–360s. The current doses were 12, 30, 60, 600 1800, 3600, 7200, 24 000 and 144000 μC. The teeth were fixed in Bouin or Baker fluids, the pulps removed, dehydrated and immersed in paraffin, then sectioned, stained with haematoxylin and eosin, and studied under a light microscope. Results Neither direct nor alternating current, similar to that applied in electrical caries diagnosis caused histological changes in the odontoblasts. Conclusions There was no difference between direct and low intensity alternating current in the response of the odontoblast.