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Development of a method to detect and quantify prostaglandin E 2 in pulpal blood from cariously exposed, vital primary molar teeth
Author(s) -
Waterhouse P. J.,
Whitworth J. M.,
Nunn J. H.
Publication year - 1999
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.1999.00244.x
Subject(s) - pulp (tooth) , molar , dentistry , coronal plane , biomedical engineering , materials science , medicine , anatomy
Aim The aim of this in vitro study was to detect and quantify an established marker of inflammation, prostaglandin E 2 (PGE 2 ), in blood samples harvested from radicular pulp stumps after coronal pulp amputation. Methodology Harvesting was achieved by a paper strip ‘dip‐stick’ method and the volume of each sample estimated before storage at − 808C. A competitive, plate‐based enzyme immunoassay technique (EIA) was developed for detection and quantification of the inflammatory mediator assumed to be present in bloodsamples. Since this technique had not previously been used to assess pulp blood, steps in the development of harvesting, storage, extraction and validation of this sensitive assay are described. Results Thirty‐nine single‐blood samples were assayed and yielded detectable amounts of PGE 2 ranging from 1.0 to 2641 ng mL − 1. Conclusions The results of this investigation indicate that the inflammatory mediator, PGE 2 can be detected and quantified in small blood samples from pulp stumps. Further development may derive quantitative tests for determining the condition of pulp tissue in primary molar pulp treatment.