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Black‐pigmented anaerobic rods in closed periapical lesions
Author(s) -
Bogen G.,
Slots J.
Publication year - 1999
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.1999.00216.x
Subject(s) - prevotella intermedia , lesion , porphyromonas gingivalis , dentistry , medicine , prevotella , saliva , endodontics , microbiology and biotechnology , periodontitis , biology , pathology , bacteria , genetics
Aim This study determined the frequency of Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens in 20 closed periapical lesions associated with symptomatic and asymptomatic refractory endodontic disease. Methodology To deliniate possible oral sources of P. endodontalis , the presence of the organism was assessed in selected subgingival sites and saliva in the same study patients. Periapical samples were obtained by paper points during surgical endodontic procedures using methods designed to minimize contamination by non‐endodontic microorganisms. Subgingival plaque samples were obtained by paper points from three periodontal pockets and from the pocket of the toothassociated with the closed periapical lesion. Unstimulated saliva was collected from the surface of the soft palate. Bacterial identification was performed using a species‐specific polymerase chain reaction (PCR) detection method. Results P. endodontalis was not identified in any periapical lesion, even though subgingival samples from eight patients (40%) revealed the P. endodontalis ‐specific amplicon. P. gingivalis occurred in one periapical lesion that was associated with moderate pain. P. nigrescens, P. endodontalis and P. intermedia were not detected in any periapical lesion studied. Conclusions Black‐pigmented anaerobic rods appear to be infrequent inhabitants of the closed periapical lesion.