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Antimicrobial effects of various endodontic irrigants on selected microorganisms
Author(s) -
Ayhan H.,
Sultan N.,
Çirak M.,
Ruhi M. Z.,
Bodur H.
Publication year - 1999
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.1999.00196.x
Subject(s) - antimicrobial , enterococcus faecalis , chlorhexidine , candida albicans , streptococcus salivarius , agar , microbiology and biotechnology , agar plate , microorganism , staphylococcus aureus , agar diffusion test , chemistry , petri dish , food science , dentistry , streptococcus mutans , biology , medicine , bacteria , genetics
Aim This study was undertaken to determine the antimicrobial effect of various endodontic irrigants against six selected microorganisms Methodology Staphylococcus aureus , Enterococcus faecalis , Streptococcus salivarius , Str. pyogenes , Escherichia coli and Candida albicans were included in the study. Pre‐sterilized Whatman paper discs, 6 mm in diameter and soaked with the test solution, were prepared and placed onto the previously seeded agar Petri plates. Each plate was incubated aerobically. A zone of inhibition was recorded for each plate and the results were analysed statistically. Results 5.25% NaOCl was effective against all test microorganisms with a substantial zone of inhibition. Saline was always ineffective. Decreased concentration of NaOCl significantly reduced its antimicrobial effect. Cresophene showed a significantly larger ( P > 0.05) average zone of inhibition compared to the other experimental irrigants. Alcohol had smaller but not significantly different zones of inhibition than chlorhexidine. Conclusions 5.25% NaOCl was superior in its antimicrobial abilities compared with other irrigants used. A reduced concentration of NaOCl (0.5%) resulted in significantly decreased antimicrobial effects. When compared with 21% alcohol, 0.5% NaOCl and 2% chlorhexidine, paramonochlorophenol (cresophene) showed a greater antimicrobial effect.