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Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers
Author(s) -
VAJRABHAYA L.,
SITHISARN P.
Publication year - 1997
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1046/j.1365-2591.1997.00056.x
Subject(s) - monolayer , root canal , cytotoxicity , absorbance , viability assay , materials science , cell culture , staining , nuclear chemistry , cell , chemistry , dentistry , chromatography , in vitro , medicine , nanotechnology , pathology , biochemistry , biology , genetics
Summary The aim of this study was to evaluate the response of a multilayer compared with a monolayer cell culture using six root canal sealers. Both monolayer and multilayer of MU‐mu‐1 (Mahidol University mouse cell line 1) were cultured in separate 96 – well plates. Following incubation at 37° in 5% CO 2 for 4 h in the presence of each sealer, cells were stained with 0.4% Sulp‐horhodamine‐B, viable dye staining and the absorbance at 540 mm determined and calculated as cell viability. There was no statistical difference in the percentage viability for each sealer in both the multilayer and the monolayer cell culture (P > 0.01). Apexit and AH‐26 were less toxic (P <0.01) than MU (Mahidol University) sealer, ROCANAL 3, ROCANAL 2, and Endomethasone which demonstrated the same toxtcity ( P >0.01)