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Characterization of acetylcholinesterases, and their genes, from the hemipteran species Myzus persicae (Sulzer), Aphis gossypii (Glover), Bemisia tabaci (Gennadius) and Trialeurodes vaporariorum (Westwood)
Author(s) -
Javed N.,
Viner R.,
Williamson M. S.,
Field L. M.,
Devonshire A. L.,
Moores G. D.
Publication year - 2003
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2003.00446.x
Subject(s) - biology , aphis gossypii , trialeurodes , myzus persicae , insect , gene , botany , methamidophos , complementary dna , locus (genetics) , acetylcholinesterase , genetics , pest analysis , homoptera , enzyme , biochemistry , pesticide , ecology , aphididae , aphid
Gene sequences encoding putative acetylcholinesterases have been reported for four hemipteran insect species. Although acetylcholinesterase insensitivity occurs in insecticide‐resistant populations of each of these species, no mutations were detected in the gene sequences from the resistant insects. This, coupled with a series of experiments using novel reversible inhibitors to compare the biochemical characteristics of acetylcholinesterase from a range of insect species, showed that the cloned cDNA fragments are unlikely to encode the hemipteran synaptic acetylcholinesterases, and there is likely to be a second ace locus.