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In vitro expression and pharmacology of the 5‐HT 7 ‐like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut‐associated nerves
Author(s) -
Lee D.W.,
Pietrantonio P. V.
Publication year - 2003
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2003.00441.x
Subject(s) - biology , aedes aegypti , octopamine (neurotransmitter) , 5 ht receptor , receptor , adenylyl cyclase , receptor expression , agonist , endocrinology , medicine , serotonin , pharmacology , biochemistry , ecology , larva
We have previously reported the cloning of a 5‐hydroxytryptamine receptor ( Aedes 5‐HT 7 ‐like receptor) from adult Aedes aegypti . For functional expression of the Aedes 5‐HT 7 ‐like receptor, CHO‐K1 cells were stably transfected with a receptor expression construct, pC5‐HT 7 . The Aedes 5‐HT 7 ‐like receptor positively coupled to Gs protein, increasing intracellular cAMP in response to 5‐HT; adenylyl cyclase activity was induced in a concentration‐dependent, saturable manner. Only 5‐HT, and not octopamine, dopamine or tyramine, caused the induction of cAMP. At 10 n m 5‐HT a weak synergism was observed between octopamine and 5‐HT. Other known agonists of the mammalian 5‐HT 7 receptor were tested. Their order of potency was: 5‐HT >> 5‐CT = 8‐OH‐DPAT >> pimozide. This is the first report on the functional expression of a mosquito neurohormone receptor.