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Characterization of JNK‐like protein derived from a mosquito cell line, C6/36
Author(s) -
Mizutani T.,
Kobayashi M.,
Eshita Y.,
Inanami O.,
Yamamori T.,
Goto A.,
Ako Y.,
Miyoshi H.,
Miyamoto H.,
Kariwa H.,
Kuwabara M.,
Takashima I.
Publication year - 2003
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2003.00387.x
Subject(s) - biology , line (geometry) , cell culture , microbiology and biotechnology , computational biology , genetics , mathematics , geometry
When Western blot analysis of heat‐killed bacteria‐ and lipopolysaccharide (LPS)‐treated Aedes albopictus mosquito cell line C6/36 was performed using antiphospholyrated c‐Jun amino‐terminal kinase (JNK) antibodies, approximately 46 kDa protein was clearly detected with a peak around 30 min. After the C6/36 cells were incubated at 45 °C in order to induce apoptosis, the 46 kDa protein continued to be detected for at least 3 h. The internalization of fluorescein‐labelled bacteria was inhibited by a JNK‐specific inhibitor SP600125, suggesting that phagocytosis involves the JNK signalling pathway in mosquito cells. Based on these results, we found one candidate for the nucleotide sequence of JNK (Ae‐JNK) from the C6/36 cells. This study is the first report regarding the mitogen‐activated protein kinase (MAPK) of mosquito.