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Linkage analysis of maternal EST cDNA clones covering all twenty‐eight chromosomes in the silkworm, Bombyx mori
Author(s) -
KadonoOkuda K.,
Kosegawa E.,
Mase K.,
Hara W.
Publication year - 2002
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2002.00353.x
Subject(s) - complementary dna , biology , bombyx mori , genetics , restriction fragment length polymorphism , bombyx , cdna library , genetic linkage , microbiology and biotechnology , linkage (software) , gene , genotype
A cDNA library of maternal messages was constructed from non‐fertilized Bombyx mori eggs collected just after oviposition without mating. cDNA clones were identified by their nucleotide sequences and evaluated as probes for RFLP linkage analysis. Back crossed F 1 segregants – by crossing an F 1 female (RF02 × RF50) and a male (RF02) – were used, which takes advantage of the phenomenon that no crossing over occurs in Bombyx females. Fifteen ordered BF 1 segregants gave either homozygous (homo) or heterozygous (hetero) RFLP patterns with each cDNA probe. cDNA probes on the same linkage group gave the same homo/hetero order. One hundred and fifty one out of 248 cDNA clones showed polymorphisms between RF02 and RF50, and were therefore suitable as probes for RFLP linkage analysis in the present BF 1 cross. They were sorted into twenty‐seven linkage groups and one independent group, by the homo/hetero pattern matrix, covering all twenty‐eight chromosomes in B. mori .