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Cloning of two cDNAs encoding isoforms of a pacifastin‐related precursor polypeptide in the desert locust, Schistocerca gregaria : analysis of stage‐ and tissue‐dependent expression
Author(s) -
Simonet G.,
Claeys I.,
November T.,
Wataleb S.,
Janssen T.,
Maes R.,
De Loof A.,
Vanden Broeck J.
Publication year - 2002
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2002.00345.x
Subject(s) - schistocerca , desert locust , biology , gene isoform , complementary dna , locust , cloning (programming) , microbiology and biotechnology , open reading frame , peptide , moulting , peptide sequence , biochemistry , gene , botany , computer science , programming language , larva
A novel serine protease inhibitor peptide family, designated as the ‘pacifastin family’, has recently been described in insects (locusts, lepidopterans) and crustaceans (crayfish). This study presents the cDNA cloning of two isoforms of SGPP‐3, a novel pacifastin‐related precursor in the desert locust, Schistocerca gregaria , which codes for three putative inhibitor peptides. The precursor isoforms differ at a single amino acid position in the third, C‐terminal peptide. Northern blot analysis confirmed the presence of two different transcripts (0.75 and 0.90 kb). Both transcripts are most abundant in the fat body and appear to be strongly regulated during the moulting cycle. In addition, the amount of transcript proved to be strictly regulated in the ovaries during the female reproductive cycle.