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High amplification of a densovirus‐derived vector in larval and adult tissues of Drosophila
Author(s) -
Royer C.,
Bossin H.,
Romane C.,
Bergoin M.,
Couble P.
Publication year - 2001
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2001.00264.x
Subject(s) - biology , genome , vector (molecular biology) , coding region , dna , virology , gene , genetics , southern blot , microbiology and biotechnology , viral vector , recombinant dna
The Lepidopteran densovirus‐derived vector, pJ lac ZΔNS3, is a defective virus genome with an insertion of lac Z DNA in the viral structural protein coding sequence, and a deletion of the sequence coding the non‐structural polypeptide NS3. pJ lac ZΔNS3 was injected into Drosophila eggs and the maintenance of the viral genome was monitored by expression of β‐galactosidase and by Southern blot hybridizations. Intense β‐galactosidase activity was observed in many somatic tissues of third‐instar larvae and adult flies, in more than 60% of the injected animals. DNA analyses showed that staining in adult tissues correlated with the amplification of the vector. Together, these results suggest the occurrence of early events of integration of the vector into the Drosophila host genome.