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Wolbachia in the Asian rice gall midge, Orseolia oryzae (Wood‐Mason): correlation between host mitotypes and infection status
Author(s) -
Behura S. K.,
Sahu S. C.,
Mohan M.,
Nair S.
Publication year - 2001
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2001.00251.x
Subject(s) - midge , gall , biology , wolbachia , mitochondrial dna , host (biology) , genetics , gene , botany , zoology
Using a PCR‐based method, we detected Wolbachia in the Asian rice gall midge. Furthermore, results showed that all females across all biotypes are infected with Wolbachia . However, all male flies are not infected and show different infection frequency in different biotypes. We have also identified three mitotypes, in the rice gall midge, based on Dra I restriction pattern of a portion of the 12S rRNA gene that was PCR amplified using primers specific to this gene. All the females and infected male flies had type 1 mtDNA while uninfected males showed only type 2 or 3 mtDNA. Inheritance patterns of mtDNA revealed the existence of a correlation between mtDNA type and Wolbachia infection in the Asian rice gall midge. Evidence for paternal inheritance of mtDNA in Wolbachia ‐free gall midge is also presented.