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Molecular characterization of ribosomal DNA polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s.
Author(s) -
Favia G.,
Lanfrancotti A.,
Spanos L.,
SidénKiamos I.,
Louis C.
Publication year - 2001
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2001.00236.x
Subject(s) - biology , genetics , anopheles gambiae , ribosomal dna , polymerase chain reaction , ribosomal rna , intergenic region , microbiology and biotechnology , nucleic acid sequence , spacer dna , ribosomal intergenic spacer analysis , dna , gene , phylogenetics , malaria , genome , immunology
The sequence of a 2.3 kb long DNA segment derived from the 5′‐most end of the ribosomal intergenic spacer was determined in three chromosomal forms of Anopheles gambiae s.s. The analysis revealed that the sequence of the Mopti form differed from that of the Bamako and Savanna forms by a total of ten nucleotide substitutions. Using these sequence variations we set up a diagnostic polymerase chain reaction (PCR) assay to distinguish mosquitoes belonging to the three chromosomal forms, facilitating studies on the distribution and the ecology of these incipient taxa. The assay also allows to distinguish whether a given specimen could represent a heterozygote between Mopti and Savanna or Bamako.