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Ultrastructural and molecular identification of a Wolbachia endosymbiont in a spider, Nephila clavata
Author(s) -
Woo Oh Hyun,
Gwang Kim Mi,
Woon Shin Sang,
Sook Bae Kyung,
Joon Ahn Young,
Park HoYong
Publication year - 2000
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2000.00218.x
Subject(s) - wolbachia , biology , bacteria , 16s ribosomal rna , proteobacteria , phylogenetic tree , phylogenetics , microbiology and biotechnology , gene , genetics , zoology
Wolbachia ‐like bacteria were observed in the egg cells of golden orb‐weaving spider, Nephila clavata , by means of transmission electron microscopy. The bacteria exhibited the typical morphology of Wolbachia , including three enveloping membranes. Based on the amplification and sequencing of partial 16S rDNA and fts Z gene, the bacteria were identified as Wolbachia , intracellular, transovarially inherited α‐proteobacteria in invertebrates. Phylogenetic analysis based on 16S rDNA and fts Z gene sequences invariably indicated that the intracellular bacteria from N. clavata belonged to group A Wolbachia , which were found only from insects. Clustering of Wolbachia from N. clavata with group A Wolbachia indicates that the bacteria were probably transferred horizontally between insects and the spider.